Index Top CAR/RXRα-binding sites in the human genoeme. rSNPs in the identified CAR/RXRα-binding sites.

rSNPs in the identified CAR/RXRα-binding sites. rSNPs in transcription factor-binding sites, which alter the ability of a transcription factor to interact with DNA, may lead to predictable differences in gene expression and may be associated with disease susceptibility. Therefore, we tried to identify rSNPs in each of the CAR/RXRα binding sequences using the NCBI SNP database. Thirty five rSNPs were identified and the effect on the binding affinity between CAR/RXRα and 35 rSNPs was examined by EMSA. As a result, 17 rSNPs were identified based on differences in the DNA-binding affinities

dbSNP Gene (Distance to TSS) Sequences Motif Concentration of competitor EMSA Competition (%) rs115089792 NONE atgGGGTCAacaaAGTTTAaga DR4 ×10 62.8 ± 0.6 atgGTGTCAacaaAGTTTAaga 36.0 ± 0.4 rs371021620 FAM188A (-543,247), PTER (-33,201) taaAGTTCAtggtaaAGTTCAtgg DR6 ×10 64.0 ± 1.1 taaAATTCAtggtaaAGTTCAtgg 19.4 ± 0.8 rs62097972 TMX3 (+168,639) tttTGAACTctgtctgaaAGGTCActt ER9 ×10 74.4 ± 0.4 tttTGAATTctgtctgaaAGGTCActt Not detected rs181870712 CSMD1 (+5,915) caaAGGTCActtaAGGTCAttt DR4 ×10 62.9 ± 1.1 caaAAGTCActtaAGGTCAttt 26.0 ± 1.5 rs117662912 CETN3 (+144,942) aggAGGTCAggaggAGTTCActg DR5 ×10 56.9 ± 0.9 aggAAGTCAggaggAGTTCActg 17.9 ± 0.6 rs113398776 SLC9A2 (+30,825), MFSD9 (+86,346) tgaAGGTCAaagAGTTTAaca DR3 ×100 95.5 ± 0.9 tgaAGGTCAaagAGATTAaca 9.5 ± 1.1 rs190307664 ESM1 (+232,659), SNX18 (+235,162) agaTGACCTctgcccgctaAGTTCAagt ER10 ×100 101.8 ± 1.5 agaTGACCTctgcccgctaACTTCAagt 36.5 ± 0.9 rs115070434 MPDZ (-24,841) taaAGGTTAaatgAGGTCActa DR4 ×100 102.8 ± 1.1 taaAGGTTAaatgAGGTCCcta 66.3 ± 5.7 rs148735323 NOX5 (-76,449), SPESP1 (+7,746) ggtGGGTCAactaAGGTCAgga DR4 ×100 97.6 ± 0.4 ggtGGATCAactaAGGTCAgga 71.1 ± 0.8 rs145145243 EYA4 (-231,989), RPS12 (+194,798) agtGGTTCAatcacAGTTCActg DR5 ×100 94.4 ± 0.9 agtGGTTCAatcacAGTTCTctg 60.7 ± 1.5 rs369662801 RTN4RL1 (+9,904), RPA1 (+185,001) tggAGCTCAgAGTTCAaag DR1 ×100 82.1 ± 0.5 tggAGCTCAgCGTTCAaag 22.1 ± 5.5 rs79536015 RPIA (+385,204) caaAGGTTAccttAGGTCAtat DR4 ×100 96.5 ± 0.2 caaAGGTTAccttAGGTCGtat 67.5 ± 0.9 rs4497728 rs4303542 rs4307980 MTRNR2L1 (+228,816) accTGAACTttcaaaggaaGGTTCAatt ER10 ×100 94.5 ± 2.1 accTGAAATttcaaaggaaGGTTCAatt 20.2 ± 0.5 accTGAACAttcaaaggaaGGTTCAatt 23.5 ± 0.6 accTGAACTttcaaaggaaGTTTCAatt 68.6 ± 0.9 rs138716773 PRL (-745,604) ttgAGCTCAattcctAGTTCAcca DR6 ×100 62.6 ± 2.4 ttgAGCTTAattcctAGTTCAcca 12.0 ± 3.9 rs11914034 ACR (+12,179), RABL2B (+33,256) caaAGGACATGAACTctt IR0 ×100 46.9 ± 0.7 caaAGGACGTGAACTctt 77.6 ± 0.5

Index Top CAR/RXRα-binding sites in the human genoeme. rSNPs in the identified CAR/RXRα-binding sites.

Kazuyuki Yanai Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan